3-isopropylmalate dehydrogenase (PA3118)

Identification
Name: 3-isopropylmalate dehydrogenase
Synonyms:
  • 3-IPM-DH
  • Beta-IPM dehydrogenase
  • IMDH
Gene Name: leuB
Enzyme Class:
Biological Properties
General Function: Involved in magnesium ion binding
Specific Function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
3-Isopropylmalate + NAD → 2-Isopropyl-3-oxosuccinate + Hydrogen ion + NADH
ReactionCard
Thumb+Thumb+Thumb+Thumb ↔ Thumb+Thumb
2-Ketobutyric acid + Carbon dioxide + NADH + Hydrogen ion ↔ D-Erythro-3-Methylmalate + NAD
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
3-Isopropylmalate + NAD ↔ 2-Isopropyl-3-oxosuccinate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+Thumb
3-Isopropylmalate + NAD ↔ 2-Isopropyl-3-oxosuccinate + NADH + Hydrogen ion
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb+Thumb+Thumb
3-Isopropylmalate + NAD + 2-Isopropyl-3-oxosuccinate ↔ Ketoleucine + Carbon dioxide + NADH + Hydrogen ion
ReactionCard
SMPDB Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
3-Isopropylmalate + NAD → NADH + Hydrogen ion + 2-Isopropyl-3-oxosuccinate
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PseudoCyc/BioCyc Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
3-Isopropylmalate + NAD → 2-Isopropyl-3-oxosuccinate + Hydrogen ion + NADH
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Thumb+Thumb ↔ Thumb+Thumb+Thumb
3-Isopropylmalate + NAD ↔ 2-Isopropyl-3-oxosuccinate + NADH + Hydrogen ion
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb+Thumb
3-Isopropylmalate + NAD → Ketoleucine + Carbon dioxide + NADH
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0008232-Isopropyl-3-oxosuccinateMetaboCard
PAMDB0000012-Ketobutyric acidMetaboCard
PAMDB0006413-IsopropylmalateMetaboCard
PAMDB000568Carbon dioxideMetaboCard
PAMDB000985D-Erythro-3-MethylmalateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000174KetoleucineMetaboCard
PAMDB000197NADMetaboCard
PAMDB000398NADHMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
3-isopropylmalate dehydrogenase activity
binding
catalytic activity
cation binding
ion binding
magnesium ion binding
metal ion binding
NAD or NADH binding
nucleotide binding
oxidoreductase activity
oxidoreductase activity, acting on CH-OH group of donors
oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
Process
branched chain family amino acid metabolic process
cellular amino acid and derivative metabolic process
cellular amino acid metabolic process
cellular metabolic process
leucine biosynthetic process
leucine metabolic process
metabolic process
oxidation reduction
Gene Properties
Locus tag: PA3118
Strand: -
Entrez Gene ID: 882812
Accession: NP_251808.1
GI: 15598314
Sequence start: 3500668
Sequence End: 3501750
Sequence Length: 1082
Gene Sequence: 
>PA3118
ATGAGCAAGCAGATCCTGGTTCTCCCCGGTGACGGTATCGGCCCGGAAATCATGGCGGAAGCGGTCAAGGTGCTGGAACTGGCCAACGACAGGTTCCAGCTCGGCTTCGAACTGGCCGAGGACGTCATCGGTGGCGCGGCCATCGACAAGCACGGCGTGCCGCTGGCCGACCAGACCCTGCAGCGCGCGCGCCAGGCCGATGCGGTGCTGCTGGGCGCGGTAGGGGGGCCGAAATGGGACAGGATCGAACGCGATATCCGTCCCGAGCGCGGTCTGCTGAAGATTCGCTCGCAACTGGGCCTGTTCGCCAACCTGCGTCCGGCCATCCTCTATCCGCAACTGGCCGCGGCCTCCAGCCTGAAGCCGGAAGTGGTCGCCGGGCTGGACATCCTCATCGTCCGCGAACTGACCGGCGGCATCTACTTCGGCCAGCCCCGCGAGCAGCGCGTGCTGGAGAACGGCGAGCGCCAGGCCTACGACACCCTGCCGTACAGCGAGAGCGAAATCCGTCGTATCGCCCGCGTCGGTTTCGACATGGCCCGCGTACGCAACAACAGGCTCTGCTCGGTGGACAAGGCCAACGTTCTCGCCTCCAGCCAACTGTGGCGGGAAGTGGTGGAAGAGGTGGCGAAGGACTACCCGGACGTCGAGCTTTCCCATATGTACGTGGATAACGCCGCCATGCAACTGGTGCGCGCGCCCAAGCAGTTCGACGTGATGGTCACCGACAACATGTTCGGCGACATCCTGTCGGATGAGGCTTCCATGCTGACCGGTTCCATCGGCATGCTGCCTTCTGCCTCGCTCGACGCGAACAACAAGGGCATGTACGAACCCTGCCACGGTTCCGCCCCGGACATCGCCGGTCAGGGCATCGCCAACCCGTTGGCGACCATCCTCTCGGTCTCGATGATGTTGCGCTACAGCTTCAGCCAGGCGACCGCCGCCGACGCCATCGAGCAGGCGGTGAGCAAGGTCCTCGATCAGGGGCTGCGCACCGGCGACATCTGGTCCGAAGGCTGCCGCAAGGTCGGCACCCGGGAAATGGGCGATGCCGTAGTCGCGGCACTCAAGAATCTGTAA
Protein Properties
Protein Residues: 360
Protein Molecular Weight: 39.1 kDa
Protein Theoretical pI: 4.77
Hydropathicity (GRAVY score): -0.067
Charge at pH 7 (predicted): -9.36
Protein Sequence: 
>PA3118
MSKQILVLPGDGIGPEIMAEAVKVLELANDRFQLGFELAEDVIGGAAIDKHGVPLADQTLQRARQADAVLLGAVGGPKWDRIERDIRPERGLLKIRSQLGLFANLRPAILYPQLAAASSLKPEVVAGLDILIVRELTGGIYFGQPREQRVLENGERQAYDTLPYSESEIRRIARVGFDMARVRNNRLCSVDKANVLASSQLWREVVEEVAKDYPDVELSHMYVDNAAMQLVRAPKQFDVMVTDNMFGDILSDEASMLTGSIGMLPSASLDANNKGMYEPCHGSAPDIAGQGIANPLATILSVSMMLRYSFSQATAADAIEQAVSKVLDQGLRTGDIWSEGCRKVGTREMGDAVVAALKNL
References
External Links:
Resource Link
Genome ID: PA3118
Entrez Gene ID: 882812
NCBI Protein ID: 15598314
General Reference: PaperBLAST - Find papers about PA3118 and its homologs