Dihydroxy-acid dehydratase (PA0353)

Identification
Name: Dihydroxy-acid dehydratase
Synonyms:
  • DAD
Gene Name: ilvD
Enzyme Class:
Biological Properties
General Function: Involved in metabolic process
Specific Function: 2,3-dihydroxy-3-methylbutanoate = 3-methyl-2- oxobutanoate + H(2)O
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb → Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate → alpha-Ketoisovaleric acid + Water
ReactionCard
Thumb → Thumb+Thumb
(R) 2,3-Dihydroxy-3-methylvalerate → 3-Methyl-2-oxovaleric acid + Water
ReactionCard
Thumb ↔ Thumb+Thumb+Thumb
2,3-Dihydroxyisovaleric acid ↔ alpha-Ketoisovaleric acid + Water + a-Ketoisovaleric acid
ReactionCard
SMPDB Reactions:
Thumb → Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate → Water + Isovaleric acid
ReactionCard
Thumb → Thumb+3-Methyl-2-oxovaleric acid+Thumb
(R) 2,3-Dihydroxy-3-methylvalerate → Water + 3-Methyl-2-oxovaleric acid + 3-Methyl-2-oxovaleric acid
ReactionCard
Thumb ↔ 2 Thumb+Thumb
2,3-Dihydroxyisovaleric acid ↔ 2 a-Ketoisovaleric acid + Water
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb → Thumb+Thumb
(R)-2,3-Dihydroxy-isovalerate → alpha-Ketoisovaleric acid + Water
ReactionCard
Thumb → Thumb+Thumb
(R) 2,3-Dihydroxy-3-methylvalerate → 3-Methyl-2-oxovaleric acid + Water
ReactionCard
Complex Reactions:
Thumb → Thumb+Thumb
2,3-Dihydroxyisovaleric acid → a-Ketoisovaleric acid + Water
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000822(R) 2,3-Dihydroxy-3-methylvalerateMetaboCard
PAMDB000589(R)-2,3-Dihydroxy-isovalerateMetaboCard
PAMDB0018752,3-Dihydroxyisovaleric acidMetaboCard
PAMDB0006052-Keto-3-methyl-valerateMetaboCard
PAMDB0001413-Methyl-2-oxovaleric acidMetaboCard
PAMDB000004a-Ketoisovaleric acidMetaboCard
PAMDB000606alpha-Ketoisovaleric acidMetaboCard
PAMDB000177Isovaleric acidMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
carbon-oxygen lyase activity
catalytic activity
dihydroxy-acid dehydratase activity
hydro-lyase activity
lyase activity
Process
branched chain family amino acid biosynthetic process
cellular amino acid and derivative metabolic process
cellular amino acid biosynthetic process
cellular amino acid metabolic process
cellular metabolic process
metabolic process
Gene Properties
Locus tag: PA0353
Strand: -
Entrez Gene ID: 879274
Accession: NP_249044.1
GI: 15595550
Sequence start: 396057
Sequence End: 397895
Sequence Length: 1838
Gene Sequence: 
>PA0353
ATGCCCGATTACCGCTCGAAAACCTCCACCCACGGCCGCAACATGGCCGGCGCCCGCGCCCTCTGGCGCGCCACCGGGATGAAGGACGAAGACTTCAAGAAGCCGATCATCGCCATCGCCAACTCCTTCACCCAGTTCGTGCCCGGCCACGTGCACCTGAAGGACCTCGGCCAACTGGTCGCCCGCGAGATCGAGAAGGCCGGCGGCGTGGCCAAGGAATTCAACACCATCGCCGTCGACGACGGCATCGCCATGGGCCACGACGGCATGCTCTATTCGCTGCCGAGCCGGGAGATCATCGCCGACTCCGTGGAATACATGGTCAACGCCCACTGCGCCGACGCCATCGTCTGCATCTCCAACTGCGACAAGATCACCCCCGGCATGCTGATGGCCGCGCTGCGCCTGAACATCCCGGTAGTGTTCGTCTCCGGCGGGCCGATGGAAGCCGGCAAGACCAAGCTGGCCAGCCACGGCCTGGACCTGGTCGATGCCATGGTGGTGGCCGCCGACGACTCCTGCTCCGACGAGAAGGTCGCCGAGTACGAGCGCAGCGCCTGCCCGACCTGCGGCTCCTGCTCCGGCATGTTCACCGCCAACTCGATGAACTGCCTGACCGAGGCGCTCGGCCTGTCGCTGCCGGGCAACGGCTCGACCCTGGCCACCCACGCCGACCGCGAGCAACTGTTCCTACGCGCCGGGCGCCTGGCCGTGGAACTCTGCCAGCGCTATTACGGCGAAGGCGACGACAGCGTGCTGCCGCGCAACATCGCCAACTTCAAGGCCTTCGAGAACGCCATGACCCTGGACATCGCCATGGGCGGCTCGACCAATACCATCCTGCACCTGCTGGCCGCCGCCCAGGAGGCCGAGGTGCCGTTCGACCTGCGCGACATCGACCGCCTGTCGCGCAAGGTGCCGCAACTGTGCAAGGTGGCGCCGAACATCCAGAAGTACCACATGGAAGACGTGCATCGCGCCGGCGGCATCTTCTCCATCCTCGGCGAACTGGCCCGCGGTGGCCTGCTGCACACCGACGTCCCCACCGTGCACAGCCCGAGCATGGCCGACGCCATCGCCCAGTGGGACATCACCCAGACCCGCGACGAAGCCGTGCACACCTTCTTCAAGGCCGGTCCGGCGGGCATCCCGACCCAGACCGCGTTCAGCCAGAACACCCGCTGGCCGAGCCTGGACGACGACCGCGCCGAGGGCTGCATCCGCAGCGTCGAGCATGCCTATTCCAAGGAAGGCGGGCTGGCCGTGCTCTACGGCAACATCGCTCTCGACGGCTGCGTGGTGAAGACCGCCGGGGTCGACGAGTCGATCCACGTGTTCGAAGGCAGCGCGAAGATCTTCGAAAGCCAGGACGCCGCGGTGAAAGGCATCCTCGGCGACGAGGTGAAGGCCGGCGACATCGTGATCATCCGCTACGAAGGACCGAAGGGCGGGCCGGGCATGCAGGAAATGCTCTATCCGACCAGCTACCTGAAGTCCAAGGGCCTGGGCAAGCAGTGCGCCCTGCTCACCGACGGACGCTTCTCCGGCGGCACCTCGGGCCTGTCCATCGGCCACGCCTCCCCGGAAGCCGCCGCCGGCGGCGCCATCGGCCTGGTGCAGGACGGCGACAAGGTGCTGATCGACATTCCCAATCGCTCGATCAACCTGCTGGTCAGCGACGAAGAACTCGCCGCACGCCGCGCCGAACAGGACAAGAAGGGCTGGAAGCCGGCCGCGCCGCGTGCCCGCCGCGTCAGCACCGCGCTCAAGGCCTACGCCCTGCTCGCCACCAGCGCCGACAAGGGCGCGGTACGCAACAAGGCGCTGCTGGACGGCTGA
Protein Properties
Protein Residues: 612
Protein Molecular Weight: 65.2 kDa
Protein Theoretical pI: 5.98
Hydropathicity (GRAVY score): -0.11
Charge at pH 7 (predicted): -9.52
Protein Sequence: 
>PA0353
MPDYRSKTSTHGRNMAGARALWRATGMKDEDFKKPIIAIANSFTQFVPGHVHLKDLGQLVAREIEKAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSREIIADSVEYMVNAHCADAIVCISNCDKITPGMLMAALRLNIPVVFVSGGPMEAGKTKLASHGLDLVDAMVVAADDSCSDEKVAEYERSACPTCGSCSGMFTANSMNCLTEALGLSLPGNGSTLATHADREQLFLRAGRLAVELCQRYYGEGDDSVLPRNIANFKAFENAMTLDIAMGGSTNTILHLLAAAQEAEVPFDLRDIDRLSRKVPQLCKVAPNIQKYHMEDVHRAGGIFSILGELARGGLLHTDVPTVHSPSMADAIAQWDITQTRDEAVHTFFKAGPAGIPTQTAFSQNTRWPSLDDDRAEGCIRSVEHAYSKEGGLAVLYGNIALDGCVVKTAGVDESIHVFEGSAKIFESQDAAVKGILGDEVKAGDIVIIRYEGPKGGPGMQEMLYPTSYLKSKGLGKQCALLTDGRFSGGTSGLSIGHASPEAAAGGAIGLVQDGDKVLIDIPNRSINLLVSDEELAARRAEQDKKGWKPAAPRARRVSTALKAYALLATSADKGAVRNKALLDG
References
External Links:
Resource Link
Genome ID: PA0353
Entrez Gene ID: 879274
NCBI Protein ID: 15595550
General Reference: PaperBLAST - Find papers about PA0353 and its homologs