3-methyl-2-oxobutanoate hydroxymethyltransferase (PA4729)

Identification
Name: 3-methyl-2-oxobutanoate hydroxymethyltransferase
Synonyms:
  • Ketopantoate hydroxymethyltransferase
  • KPHMT
Gene Name: panB
Enzyme Class:
Biological Properties
General Function: Involved in 3-methyl-2-oxobutanoate hydroxymethyltransferase activity
Specific Function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate
Cellular Location: Cytoplasm (Potential)
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb+Thumb+Thumb ↔ Thumb+Thumb
alpha-Ketoisovaleric acid + Water + 5,10-Methylene-THF + a-Ketoisovaleric acid ↔ 2-Dehydropantoate + Tetrahydrofolic acid
ReactionCard
Thumb+Thumb+Thumb ↔ Thumb+Thumb
5,10-Methylene-THF + alpha-Ketoisovaleric acid + Water ↔ Tetrahydrofolic acid + 2-Dehydropantoate
ReactionCard
SMPDB Reactions:
Thumb+5,10-Methylene-THF+Thumb+Thumb → Tetrahydrofolic acid+2-dehydropantoate+Thumb+Thumb
a-Ketoisovaleric acid + 5,10-Methylene-THF + Water + 5,10-Methylene-THF → Tetrahydrofolic acid + 2-dehydropantoate + Tetrahydrofolic acid + 2-Dehydropantoate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Thumb+Thumb ↔ Thumb+Thumb
alpha-Ketoisovaleric acid + Water + 5,10-Methylene-THF + a-Ketoisovaleric acid ↔ 2-Dehydropantoate + Tetrahydrofolic acid
ReactionCard
Complex Reactions:
Thumb+Thumb+Thumb → Thumb+Thumb
5,10-Methylene-THF + a-Ketoisovaleric acid + Water → Tetrahydrofolic acid + 2-Dehydropantoate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0005762-DehydropantoateMetaboCard
PAMDB0003535,10-Methylene-THFMetaboCard
PAMDB000004a-Ketoisovaleric acidMetaboCard
PAMDB000606alpha-Ketoisovaleric acidMetaboCard
PAMDB000424Tetrahydrofolic acidMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
3-methyl-2-oxobutanoate hydroxymethyltransferase activity
catalytic activity
glycine hydroxymethyltransferase activity
hydroxymethyl-, formyl- and related transferase activity
methyltransferase activity
transferase activity
transferase activity, transferring one-carbon groups
Process
cellular metabolic process
coenzyme biosynthetic process
coenzyme metabolic process
cofactor metabolic process
metabolic process
pantothenate biosynthetic process
Gene Properties
Locus tag: PA4729
Strand: +
Entrez Gene ID: 881621
Accession: NP_253417.1
GI: 15599923
Sequence start: 5311464
Sequence End: 5312264
Sequence Length: 800
Gene Sequence: 
>PA4729
ATGCCTGATGTAACCCTGACCACCCTGCAAGGGTTGAAGCAGAGCGGCGAGAAGATCGCCATGCTGACCTGCTACGACGCCACCTTCGCCCACACCGCCAGCCAGGCCGGCGTGGACGTGCTTCTGGTCGGAGATTCGCTCGGAATGGTATTGCAGGGTCACGACAGCACCCTGCCGGTCAGCAACGAGGAAATGGCCTATCACACGGCCTGCGTGAAACGCGGTAACAAAGGTTCCCTGATCGTCACCGACCTGGCCTTCGAATCCAGCCATTCCGTGGCCCAGACCCTTGCCGACGCGGTCCGCCTGATGCAGGCCGGCGCACACATGGTCAAGCTGGAGGGTGGGGTCTGGCTGGCGGAGCCGATCGCCCGCCTGGCACAGATGGGCGTGCCGGTCTGCGCGCATCTCGGCCTGACCCCGCAGGCGGTCAACCTGTTCGGCGGCTTCAAGGTCCAGGGCCGCCAGGAAACCCAGGCGCGCCAGTTGCGCGCCGATGCCATCGCCCTGGAGCAGGCCGGCGCCGCCATGCTGCTGCTGGAGTGCGTGCCCAGCGTGCTGGCCGAAGAGATCACCCAGGCGGTGAAGATCCCGGTGATCGGGATTGGCGCCGGTGCGGCGACCGATGGCCAGGTGCTGGTCATGCACGACATGCTCGGTCTCTCGCTGACCGGCCGCTCGCCCAAGTTCGTCAAGGATTTCATGCAAGGCCAGGAGAGCATCCCGGCCGCCATCGCGGCCTATGTCCGCGCCGTCAAGGACGTGTCCTTCCCCGCCGCCGAACACGGATTCAACGCATGA
Protein Properties
Protein Residues: 266
Protein Molecular Weight: 27.9 kDa
Protein Theoretical pI: 5.82
Hydropathicity (GRAVY score): 0.271
Charge at pH 7 (predicted): -5.2
Protein Sequence: 
>PA4729
MPDVTLTTLQGLKQSGEKIAMLTCYDATFAHTASQAGVDVLLVGDSLGMVLQGHDSTLPVSNEEMAYHTACVKRGNKGSLIVTDLAFESSHSVAQTLADAVRLMQAGAHMVKLEGGVWLAEPIARLAQMGVPVCAHLGLTPQAVNLFGGFKVQGRQETQARQLRADAIALEQAGAAMLLLECVPSVLAEEITQAVKIPVIGIGAGAATDGQVLVMHDMLGLSLTGRSPKFVKDFMQGQESIPAAIAAYVRAVKDVSFPAAEHGFNA
References
External Links:
Resource Link
Genome ID: PA4729
Entrez Gene ID: 881621
NCBI Protein ID: 15599923
General Reference: PaperBLAST - Find papers about PA4729 and its homologs