Identification
Name: 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD
Synonyms:
  • N-acetylmuramoyl-L-alanine amidase
Gene Name: ampD
Enzyme Class:
Biological Properties
General Function: Involved in N-acetylmuramoyl-L-alanine amidase activity
Specific Function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions: Not Available
SMPDB Reactions:
GlcNAc-1,6-anhMurNAc-L-Ala-?-D-Glu-DAP-D-Ala+ThumbN-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate+L-Ala-gamma-D-Glu-DAP-D-Ala
GlcNAc-1,6-anhMurNAc-L-Ala-?-D-Glu-DAP-D-Ala + Water → N-acetyl-β-D-glucosamine(anhydrous)-N-acetylmuramate + L-Ala-gamma-D-Glu-DAP-D-Ala
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb
a β-lactam + a β-lactam + H2O = a substituted β-amino acid
ReactionCard
PAMDB120226+PAMDB000142PAMDB120237+PAMDB120146
Complex Reactions:
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Thumb+ThumbThumb+Thumb
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0013271,6-Anhydro-N-acetylmuramateMetaboCard
PAMDB0015201,6-Anhydrous-N-Acetylmuramyl-tetrapeptideMetaboCard
PAMDB0015211,6-Anhydrous-N-Acetylmuramyl-tripeptideMetaboCard
PAMDB001648L-alanine-D-glutamate-meso-2,6-diaminoheptanedioateMetaboCard
PAMDB001647L-Alanine-D-glutamate-meso-2,6-diaminoheptanedioate-D-alanineMetaboCard
PAMDB001656N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramic acidMetaboCard
PAMDB001657N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tetrapeptideMetaboCard
PAMDB001658N-Acetyl-D-glucosamine(anhydrous)N-Acetylmuramyl-tripeptideMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
catalytic activity
hydrolase activity
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds
hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides
N-acetylmuramoyl-L-alanine amidase activity
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan catabolic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process
Gene Properties
Locus tag: PA4522
Strand: -
Entrez Gene ID: 881105
Accession: NP_253212.1
GI: 15599718
Sequence start: 5064774
Sequence End: 5065340
Sequence Length: 566
Gene Sequence:
>PA4522
ATGCATTTCGATTCCGTTACCGGCTGGGTCCGTGGCGTCCGCCACTGCCCTTCACCGAATTTCAACCTGCGTCCGCAAGGGGACGCGGTGTCGCTGCTGGTTATCCACAATATCAGCCTGCCGCCGGGACAGTTCGGCACCGGTAAGGTCCAGGCGTTCTTCCAGAATCGCCTGGACCCGAACGAACATCCCTATTTCGAGGAGATCCGCCACCTGACGGTTTCCGCGCATTTCCTCATCGAACGCGACGGCGCCATCACCCAGTTCGTCTCCTGTCACGACCGCGCCTGGCATGCCGGGGTTTCCTGCTTCGACGGTCGCGAGGCGTGCAACGACTTCTCCCTTGGCATCGAACTGGAGGGGACCGACACGGAACCGTACACGGACGCGCAGTACACTGCCCTGGCCGGGCTGACGCGGCTGTTGCGCGCGGCCTTCCCGGGGATCACCCCGGAACGCATCCAGGGCCACTGCGATATCGCCCCGGAGCGCAAGACCGATCCCGGCGAGGCTTTCGACTGGTCGCGCTACCGCGCCGGATTGACCGATTCCAAGGAGGAAACATGA
Protein Properties
Protein Residues: 188
Protein Molecular Weight: 21 kDa
Protein Theoretical pI: 5.5
Hydropathicity (GRAVY score): -0.447
Charge at pH 7 (predicted): -6.99
Protein Sequence:
>PA4522
MHFDSVTGWVRGVRHCPSPNFNLRPQGDAVSLLVIHNISLPPGQFGTGKVQAFFQNRLDPNEHPYFEEIRHLTVSAHFLIERDGAITQFVSCHDRAWHAGVSCFDGREACNDFSLGIELEGTDTEPYTDAQYTALAGLTRLLRAAFPGITPERIQGHCDIAPERKTDPGEAFDWSRYRAGLTDSKEET
References
External Links:
Resource Link
Genome ID: PA4522
Entrez Gene ID: 881105
NCBI Protein ID: 15599718
General Reference: PaperBLAST - Find papers about PA4522 and its homologs