P. aeruginosa Metabolome Database Protein PA4415

Identification

Name:

Phospho-N-acetylmuramoyl-pentapeptide-transferase

Synonyms:

UDP-MurNAc-pentapeptide phosphotransferase

Gene Name:

mraY

Enzyme Class:

2.7.8.13

Biological Properties

General Function:

Involved in phospho-N-acetylmuramoyl-pentapeptide-transferase activity

Specific Function:

First step of the lipid cycle reactions in the biosynthesis of the cell wall peptidoglycan

Cellular Location:

Cell inner membrane; Multi-pass membrane protein

KEGG Pathways:

Metabolic pathways pae01100
Peptidoglycan biosynthesis pae00550

KEGG Reactions:

SMPDB Reactions:

di-trans,octa-cis-undecaprenyl phosphate

→

Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine

UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + di-trans,octa-cis-undecaprenyl phosphate → Uridine 5'-monophosphate + Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine
ReactionCard

di-trans,octa-cis-undecaprenyl phosphate

→

N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine + di-trans,octa-cis-undecaprenyl phosphate → N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol + Uridine 5'-monophosphate
ReactionCard

PseudoCyc/BioCyc Reactions:

→

UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine + Di-trans,poly-cis-undecaprenyl phosphate → N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol + Uridine 5'-monophosphate
ReactionCard

Complex Reactions:

→

Undecaprenyl phosphate + UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine → Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine + Uridine 5'-monophosphate
ReactionCard

Transports:

Not Available

Metabolites:

PAMDB ID	Name	View
PAMDB001145	Di-trans,poly-cis-undecaprenyl phosphate	MetaboCard
PAMDB003420	Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol	MetaboCard
PAMDB001020	MurAc(oyl-L-Ala-D-gamma-Glu-L-Lys-D-Ala-D-Ala)-diphospho-undecaprenol	MetaboCard
PAMDB001129	N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine-diphosphoundecaprenyl-N-acetylglucosamine	MetaboCard
PAMDB001100	N-Acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol	MetaboCard
PAMDB003528	UDP-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)	MetaboCard
PAMDB006368	UDP-N-acetyl-α-D-muramoyl-L-alanyl-γ-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine	MetaboCard
	UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-6-carboxy-L-lysyl-D-alanyl-D-alanine	MetaboCard
PAMDB001051	UDP-N-Acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine	MetaboCard
PAMDB001054	Undecaprenyl phosphate	MetaboCard
PAMDB001056	Undecaprenyl-diphospho-N-acetylmuramoyl-(N-acetylglucosamine)-L-alanyl-D-glutaminyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine	MetaboCard
PAMDB001062	Undecaprenyl-diphospho-N-acetylmuramoyl-L-alanyl-D-glutamyl-meso-2,6-diaminopimeloyl-D-alanyl-D-alanine	MetaboCard
PAMDB000118	Uridine 5'-monophosphate	MetaboCard

GO Classification:

Component
cell part
integral to membrane
intrinsic to membrane
membrane
membrane part
Function
catalytic activity
phospho-N-acetylmuramoyl-pentapeptide-transferase activity
phosphotransferase activity, for other substituted phosphate groups
transferase activity
transferase activity, transferring phosphorus-containing groups
Process
aminoglycan metabolic process
carbohydrate metabolic process
glycosaminoglycan metabolic process
metabolic process
peptidoglycan biosynthetic process
peptidoglycan metabolic process
polysaccharide metabolic process
primary metabolic process

Gene Properties

Locus tag:

PA4415

Strand:

Entrez Gene ID:

881288

Accession:

NP_253105.1

GI:

15599611

Sequence start:

4948690

Sequence End:

4949772

Sequence Length:

1082

Gene Sequence:

>PA4415
ATGCTCCTGCTGCTGGCCGAATACCTGCAACAGTTCTACAAGGGCTTCGGCGTCTTCCAGTACCTGACCCTGCGCGGCATTCTCAGCGTGCTCACCGCGCTGTCGCTGTCGCTGTGGCTGGGGCCCTGGATGATCCGTACCTTGCAGATCCGCCAGATCGGCCAGGCCGTGCGCAACGACGGTCCGCAGTCGCACCTGTCGAAGAAGGGCACCCCGACCATGGGCGGCGCCCTGATCCTTACCGCCATAGCCATCAGCACGCTGCTGTGGGCGGATCTTTCCAACCGCTACGTGTGGGTAGTGCTGGTCGTTACCCTGCTGTTCGGTGCCATCGGCTGGGTGGACGACTACCGCAAGGTGATCGAGAAGAACTCCCGTGGCCTGCCGAGCCGCTGGAAGTACTTCTGGCAGTCGGTGTTCGGCATCGGCGCCGCCGTGTTCCTCTACATGACTGCCGAAACCCCGATCGAGACCACCCTGATCGTGCCGATGCTGAAGAGCGTCGAGATCCAGTTGGGCATCTTCTTCGTGGTCCTGACCTACTTCGTCATCGTCGGCTCGAGCAATGCGGTGAACCTCACCGACGGTCTCGACGGCCTGGCGATCATGCCGACGGTAATGGTTGCCGGCGCGCTGGGCATCTTCTGCTACCTGTCGGGCAACGTGAAGTTCGCCGAGTACCTGCTGATTCCCAACGTACCGGGCGCCGGCGAGCTGATCGTGTTCTGCGCCGCGCTGGTCGGCGCCGGCCTCGGCTTCCTCTGGTTCAACACCTATCCGGCGCAGGTCTTCATGGGCGACGTCGGCGCGCTGGCGCTGGGCGCCGCGCTGGGCACCATCGCGGTGATCGTGCGCCAGGAGATCGTGCTGTTCATCATGGGTGGGGTGTTCGTCATGGAAACCCTCTCGGTGATGATCCAGGTCGCTTCCTTCAAGCTGACCGGACGCCGCGTCTTCCGTATGGCGCCGATCCATCACCATTTCGAACTGAAAGGCTGGCCGGAGCCGCGCGTGATCGTGCGCTTCTGGATCATCACCGTGATCCTGGTGCTGATCGGCCTCGCCACCTTGAAGCTGCGTTGA

Protein Properties

Protein Residues:

360

Protein Molecular Weight:

39.7 kDa

Protein Theoretical pI:

9.85

Hydropathicity (GRAVY score):

0.823

Charge at pH 7 (predicted):

8.88

Protein Sequence:

>PA4415
MLLLLAEYLQQFYKGFGVFQYLTLRGILSVLTALSLSLWLGPWMIRTLQIRQIGQAVRNDGPQSHLSKKGTPTMGGALILTAIAISTLLWADLSNRYVWVVLVVTLLFGAIGWVDDYRKVIEKNSRGLPSRWKYFWQSVFGIGAAVFLYMTAETPIETTLIVPMLKSVEIQLGIFFVVLTYFVIVGSSNAVNLTDGLDGLAIMPTVMVAGALGIFCYLSGNVKFAEYLLIPNVPGAGELIVFCAALVGAGLGFLWFNTYPAQVFMGDVGALALGAALGTIAVIVRQEIVLFIMGGVFVMETLSVMIQVASFKLTGRRVFRMAPIHHHFELKGWPEPRVIVRFWIITVILVLIGLATLKLR

References

External Links:

Resource	Link
Genome ID:	PA4415
Entrez Gene ID:	881288
NCBI Protein ID:	15599611

General Reference:

PaperBLAST - Find papers about PA4415 and its homologs

Phospho-N-acetylmuramoyl-pentapeptide-transferase (PA4415)