Glycerol-3-phosphate acyltransferase (PA3673)

Identification
Name: Glycerol-3-phosphate acyltransferase
Synonyms:
  • GPAT
Gene Name: plsB
Enzyme Class:
Biological Properties
General Function: Involved in transferase activity, transferring acyl groups
Specific Function: Catalyzes the transfer of an acyl group from acyl-ACP to glycerol-3-phosphate (G3P) to form lysophosphatidic acid (LPA). This enzyme can utilize either acyl-CoA or acyl-ACP as the fatty acyl donor
Cellular Location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side
KEGG Pathways:
KEGG Reactions:
Thumb+Acyl-CoA+Acyl-CoA ↔ 1-Acyl-sn-glycerol 3-phosphate+Thumb+1-Acyl-sn-glycerol 3-phosphate
Glycerol 3-phosphate + Acyl-CoA + Acyl-CoA ↔ 1-Acyl-sn-glycerol 3-phosphate + Coenzyme A + 1-Acyl-sn-glycerol 3-phosphate
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Acyl-[acyl-carrier protein]+Thumb ↔ 1-Acyl-sn-glycerol 3-phosphate+Acyl-carrier protein
Acyl-[acyl-carrier protein] + Glycerol 3-phosphate ↔ 1-Acyl-sn-glycerol 3-phosphate + Acyl-carrier protein
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SMPDB Reactions:
acyl-[acp] +Thumb → a holo-[acyl-carrier protein]+Thumb
acyl-[acp] + Glycerol 3-phosphate → a holo-[acyl-carrier protein] + LPA(18:0/0:0)
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long-chain fatty acyl-CoA +Thumb → Thumb+Thumb
long-chain fatty acyl-CoA + Glycerol 3-phosphate → Coenzyme A + LPA(18:0/0:0)
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Thumb+Thumb → Thumb+Thumb
Glycerol 3-phosphate + Octadecenoyl-CoA (N-C18:1CoA) → LPA(18:1(9Z)/0:0) + Coenzyme A
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Thumb+acyl-[acp] → a holo-[acyl-carrier protein]?+Thumb
Glycerol 3-phosphate + acyl-[acp] → a holo-[acyl-carrier protein]? + LPA(18:1(9Z)/0:0)
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PseudoCyc/BioCyc Reactions:
a long-chain acyl-CoA+Thumb → a 1-acyl-sn-glycerol-3-phosphate+Thumb
a long-chain acyl-CoA + Glycerol 3-phosphate → a 1-acyl-sn-glycerol-3-phosphate + Coenzyme A
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Complex Reactions:
Dodecanoyl-ACP (n-C12:0ACP)+Thumb → Thumb+acyl carrier protein
Dodecanoyl-ACP (n-C12:0ACP) + Glycerol 3-phosphate → 1-Dodecanoyl-sn-glycerol 3-phosphate + acyl carrier protein
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Thumb+cis-hexadec-9-enoyl-[acyl-carrier protein] (n-C16:1) → Thumb+acyl carrier protein
Glycerol 3-phosphate + cis-hexadec-9-enoyl-[acyl-carrier protein] (n-C16:1) → 1-Hexadec-9-enoyl-sn-glycerol 3-phosphate + acyl carrier protein
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Thumb+cis-octadec-11-enoyl-[acyl-carrier protein] (n-C18:1) → Thumb+acyl carrier protein
Glycerol 3-phosphate + cis-octadec-11-enoyl-[acyl-carrier protein] (n-C18:1) → 1-Octadec-11-enoyl-sn-glycerol 3-phosphate + acyl carrier protein
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Thumb+cis-tetradec-7-enoyl-[acyl-carrier protein] (n-C14:1) → Thumb+acyl carrier protein
Glycerol 3-phosphate + cis-tetradec-7-enoyl-[acyl-carrier protein] (n-C14:1) → 1-Tetradec-7-enoyl-sn-glycerol 3-phosphate + acyl carrier protein
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Thumb+Myristoyl-ACP (n-C14:0ACP) → Thumb+acyl carrier protein
Glycerol 3-phosphate + Myristoyl-ACP (n-C14:0ACP) → 1-Tetradecanoyl-sn-glycerol 3-phosphate + acyl carrier protein
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Thumb+Octadecanoyl-ACP (n-C18:0ACP) → Thumb+acyl carrier protein
Glycerol 3-phosphate + Octadecanoyl-ACP (n-C18:0ACP) → 1-Octadecanoyl-sn-glycerol 3-phosphate + acyl carrier protein
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Thumb+Palmitoyl-ACP (n-C16:0ACP) → Thumb+acyl carrier protein
Glycerol 3-phosphate + Palmitoyl-ACP (n-C16:0ACP) → 1-hexadecanoyl-sn-glycerol 3-phosphate + acyl carrier protein
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Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0015361-Dodecanoyl-sn-glycerol 3-phosphateMetaboCard
PAMDB0015371-Hexadec-9-enoyl-sn-glycerol 3-phosphateMetaboCard
PAMDB0015381-hexadecanoyl-sn-glycerol 3-phosphateMetaboCard
PAMDB0015391-Octadec-11-enoyl-sn-glycerol 3-phosphateMetaboCard
PAMDB0015401-Octadecanoyl-sn-glycerol 3-phosphateMetaboCard
PAMDB0015411-Tetradec-7-enoyl-sn-glycerol 3-phosphateMetaboCard
PAMDB0015421-Tetradecanoyl-sn-glycerol 3-phosphateMetaboCard
PAMDB000381Coenzyme AMetaboCard
PAMDB000049Glycerol 3-phosphateMetaboCard
PAMDB006218LPA(18:0/0:0)MetaboCard
PAMDB006219LPA(18:1(9Z)/0:0)MetaboCard
PAMDB001668Octadecenoyl-CoA (N-C18:1CoA)MetaboCard
GO Classification:
Function
acyltransferase activity
catalytic activity
glycerol-3-phosphate O-acyltransferase activity
O-acyltransferase activity
transferase activity
transferase activity, transferring acyl groups
transferase activity, transferring acyl groups other than amino-acyl groups
Process
metabolic process
organophosphate metabolic process
phospholipid biosynthetic process
phospholipid metabolic process
Gene Properties
Locus tag: PA3673
Strand: +
Entrez Gene ID: 880591
Accession: NP_252363.1
GI: 15598869
Sequence start: 4112285
Sequence End: 4114789
Sequence Length: 2504
Gene Sequence: 
>PA3673
ATGCCCCGCTACCCTTTCCGCCGCTTTGGTTTTGGCGCCCTGCGCCGCCTGCTGTACCTCTGGGTACGCTCGGAAACCATCAACCAATCGGCCTTCACCCTGAAGATCGACCGCAGCAAGCCGGTGCTCTACGTGCTGCAACAGCCTTCGGTCAGCGATCTCGCGGTAGTGGATACCGAATGCCGCAAGGCCGGGCTGCCGCGTCCGGTGATGCCGGTAGCGGTAGGCGACGCCATCGAGCCGGCGGCGTTCTTCTACCTGACGCCCGAACCCGACTGGCTCGGCCGCCAGGATAAGCGCGGCGCCTCGCCGACCCTGGTGCGCATGCTCGCCGCGGTCGGCCAGAACGGTCTCGACGACGCGCAGATCATTCCGGTCAGCGTGTTCTGGGGACAATCGCCGGACAGCGAATCGAGCCCCTGGAAGCTGCTGTTCGCCGACAACTGGGCGGTCACCGGGCGCCTGCGCAAGCTGGCGCGCATCCTGATCCTCGGACGCAAGACCCGTGTGCAGTTCTCCGCGCCGATCCACCTGCGCGAGCTGGTGGAACAGGGCAAGGGCCACGAGCGCACGCTGCGCATGGTCAACCGTATCCTCCGGGTGCACTTCCGCAACCTGAAGACCGCGGTGATCGGTCCCGACCTGTCGCACCGCCGCAACCTGGTCAAGGGCCTGCTGCGCGCGCCGCTGGTACGCCAGGCGATCAGCGAGGAATGCGAGAGCGAACGGATATCCCAGGAAAAGGCCGAAGGCATCGCACTGCGCTACGCCAATGAAATCGCCTCGGACTTCTCCTATCCGGTGATCCGCTTCCTCGAAGTCATCCTCAGCTGGTTCTGGAACAAGCTGTACGAAGGGGTCAAGGTCAACCACATCGAGCGGGTGCAGGACGTGGCCCAGGGCAACGAGATCGTCTACGTGCCCTGCCACCGCAGCCACATCGACTACCTGCTGCTCTCCTACCTGCTGTTCCGCAACGGCCTGACCCCGCCGCACATCGCCGCCGGGATCAACCTGAACATGCCGGTGATCGGCTCGATCCTGCGCCGCGGCGGGGCCTTCTTCATGCGCCGCAGCTTCAAGGGCAACCAGCTCTACACCGCGGTGTTCAACGAGTACCTGCACACCCTGTTCTCCCGCGGCTTCTCCACCGAGTACTTCGTCGAGGGCGGCCGTTCGCGCACCGGGCGCATGCTCCACCCACGCACCGGCATGCTCGCCATCACCCTGCGCAGCTTCCTCCGTGACTCGCGCCGGCCGATCGTCTTCGTGCCGGTCTACATCGGCTACGAGCGCGTGCTGGAAGGCCGCACCTACCTCGGCGAACTGCGCGGCGCGACCAAGAAGAAGGAGTCGATCTTCGACCTGTTCAAGGTGGTCGGCGCCCTGAAGCAGCGTTTTGGCCAGGTCTGGGTGAACTTCGGCGAGCCGATCCATCTCGACCAGTTCCTCGACCGCCACCAGCCCGACTGGCAGGACCAGGACCTCGGCCCGGAATACCGCCCCGACTGGCTGCCGCAGACCACCAACCTGCTGGCCAAGGACGTCGCCCGCCACCTCAACGACGCGGCGGCGATCAACCCGGTCAACCTGGTCGCCCTGGCGCTGCTCTCCACCAGCCGCCAGGCCCTCGACGAAAGCGCCCTGGCGCGAATCCTCGACCTCTACCTGGCGTTGCTGCGCAAGGTGCCCTACTCGCCCAGCGCGACCCTGCCGGACGGCGACGGCCAGGCCCTGATCGAATACGTGAAGAGCATGAACCTGCTGGCCGAGCAGAAGGACGCGCTGGGCCGCATCCTCTATCTCGACGAACAGAACGCGGTGCTGGCGACCTACTACCGCAACAACGTCCTCCACGTCTTCGCCCTGCCGGCGCTGATCGCCAGCTTCTTCCAGAGCAACTCGCGGATCAGCCGCGAACAGTTGCTGCGCTTCGCCCGCGCGCTCTATCCCTACCTGCAGGCGGAACTGTTCATCCGCTGGAGCCTGGACGAACTCGACGCGGTGATCGACCAGTGGCTGGCGGCCCTGGTGGAACAGGACCTGCTGCGCCAGGAGAACGACACCTTCATCCGCCCGGCGCCAAGCTCGCGGCAATATGTGCTGCTGATCCTCCTCGCTCGTTCGGTGACCCAGACCCTGCAGCGCTTCTACATGGCCATCGCCCTGCTGCTCAACGCCGGGCAGAACGCCCTGACCGCCGAGGAACTGGAGAACCTCTGCACCGTGATGGCCCAGCGCCTGTCGATCCTGCATGGCCTGAACGCCCCGGAGTTCTTCGACAAGAGCCTGTTCCGCCACTTCATCCAGACCCTTCTCGACCTGCGCGTGCTGCGCAAGGACGAGGCCGGCAAGCTGAGCTACCACGAACTGCTCGGCGAACTGGCCGAAGGCGCCGCCAAGCGCGTGCTGCCGGCGGAGATCCGTCTGTCGATCCGCCAGGTGGCGCTGGAGCGCCCCGCCGAGGAAGCCGCCGCCGAAAGCAACGACGCGGCCGCCAACTGA
Protein Properties
Protein Residues: 834
Protein Molecular Weight: 94.8 kDa
Protein Theoretical pI: 9.66
Hydropathicity (GRAVY score): -0.097
Charge at pH 7 (predicted): 18
Protein Sequence: 
>PA3673
MPRYPFRRFGFGALRRLLYLWVRSETINQSAFTLKIDRSKPVLYVLQQPSVSDLAVVDTECRKAGLPRPVMPVAVGDAIEPAAFFYLTPEPDWLGRQDKRGASPTLVRMLAAVGQNGLDDAQIIPVSVFWGQSPDSESSPWKLLFADNWAVTGRLRKLARILILGRKTRVQFSAPIHLRELVEQGKGHERTLRMVNRILRVHFRNLKTAVIGPDLSHRRNLVKGLLRAPLVRQAISEECESERISQEKAEGIALRYANEIASDFSYPVIRFLEVILSWFWNKLYEGVKVNHIERVQDVAQGNEIVYVPCHRSHIDYLLLSYLLFRNGLTPPHIAAGINLNMPVIGSILRRGGAFFMRRSFKGNQLYTAVFNEYLHTLFSRGFSTEYFVEGGRSRTGRMLHPRTGMLAITLRSFLRDSRRPIVFVPVYIGYERVLEGRTYLGELRGATKKKESIFDLFKVVGALKQRFGQVWVNFGEPIHLDQFLDRHQPDWQDQDLGPEYRPDWLPQTTNLLAKDVARHLNDAAAINPVNLVALALLSTSRQALDESALARILDLYLALLRKVPYSPSATLPDGDGQALIEYVKSMNLLAEQKDALGRILYLDEQNAVLATYYRNNVLHVFALPALIASFFQSNSRISREQLLRFARALYPYLQAELFIRWSLDELDAVIDQWLAALVEQDLLRQENDTFIRPAPSSRQYVLLILLARSVTQTLQRFYMAIALLLNAGQNALTAEELENLCTVMAQRLSILHGLNAPEFFDKSLFRHFIQTLLDLRVLRKDEAGKLSYHELLGELAEGAAKRVLPAEIRLSIRQVALERPAEEAAAESNDAAAN
References
External Links:
Resource Link
Genome ID: PA3673
Entrez Gene ID: 880591
NCBI Protein ID: 15598869
General Reference: PaperBLAST - Find papers about PA3673 and its homologs