4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (PA3803)

Identification
Name: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase
Synonyms:
  • 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase
  • Protein gcpE
  • Protein E
Gene Name: ispG
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+2 Reduced ferredoxin+Oxidized ferredoxin ↔ Thumb+Thumb+2 Oxidized ferredoxin+Reduced ferredoxin
2-C-Methyl-D-erythritol-2,4-cyclodiphosphate + 2 Reduced ferredoxin + Oxidized ferredoxin ↔ 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + Water + 2 Oxidized ferredoxin + Reduced ferredoxin
ReactionCard
SMPDB Reactions:
Thumb+a reduced flavodoxin → Thumb+Thumb+an oxidized flavodoxin+Thumb
2-C-Methyl-D-erythritol-2,4-cyclodiphosphate + a reduced flavodoxin → Water + Hydrogen ion + an oxidized flavodoxin + 1-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb+Oxidized-ferredoxins ← Thumb+Thumb+Reduced-ferredoxins
1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + Water + Oxidized-ferredoxins ← 2-C-Methyl-D-erythritol-2,4-cyclodiphosphate + Hydrogen ion + Reduced-ferredoxins
ReactionCard
Complex Reactions:
Thumb+2 Flavodoxin reduced+Thumb → 2 flavodoxin semi oxidized+Thumb+Thumb
2-C-Methyl-D-erythritol-2,4-cyclodiphosphate + 2 Flavodoxin reduced + Hydrogen ion → 2 flavodoxin semi oxidized + 1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + Water
ReactionCard
Thumb+Thumb+2 oxidized ferredoxin → Thumb+2 reduced ferredoxin
1-Hydroxy-2-methyl-2-butenyl 4-diphosphate + Water + 2 oxidized ferredoxin → 2-C-Methyl-D-erythritol 2,4-cyclodiphosphate + 2 reduced ferredoxin
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0018681-Hydroxy-2-methyl-2-(E)-butenyl 4-diphosphateMetaboCard
PAMDB0008691-Hydroxy-2-methyl-2-butenyl 4-diphosphateMetaboCard
PAMDB0035022-C-Methyl-D-erythritol 2,4-cyclodiphosphateMetaboCard
PAMDB0006112-C-Methyl-D-erythritol-2,4-cyclodiphosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Function
4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase activity
binding
catalytic activity
cation binding
ion binding
iron ion binding
metal ion binding
oxidoreductase activity
oxidoreductase activity, acting on CH or CH2 groups
oxidoreductase activity, acting on CH or CH2 groups, with an iron-sulfur protein as acceptor
transition metal ion binding
Process
cellular lipid metabolic process
cellular metabolic process
isoprenoid biosynthetic process
isoprenoid metabolic process
lipid metabolic process
metabolic process
oxidation reduction
primary metabolic process
terpenoid biosynthetic process
terpenoid metabolic process
Gene Properties
Locus tag: PA3803
Strand: -
Entrez Gene ID: 879053
Accession: NP_252492.1
GI: 15598998
Sequence start: 4262378
Sequence End: 4263493
Sequence Length: 1115
Gene Sequence: 
>PA3803
ATGAGCATTCATAGCGCTTCTCCGATCATTCGCCGCAAGTCTCGGAAAATCTGGGTCGGCAACGTCCCGGTGGGCGGCGATGCGCCGATCGCCGTGCAGAGCATGACCAACACCGAGACCTGCGACGTCGCTGCCACCGTCGCGCAGATCCGCCGCCTGGAAGATGCCGGCGCGGACATCGTGCGGGTTTCCGTCCCCGACATGGACGCCGCCGAGGCATTCGGCAAGATCAAGCAGCAGGTCAACGTGCCGCTGGTCGCCGACATCCACTTCGACTATCGCATCGCCCTGCGCGTCGCCGAGCTGGGAGTGGACTGCCTGCGCATCAATCCGGGCAACATCGGTCGCGAGGACCGGGTCAAGGCCGTGGTCGATGCCGCGCGCGAGCGCAACATCCCGATCCGTATCGGCGTCAATGCCGGTTCGCTGGAAAAGGACCTGCAGAAGAAATACGGCGAACCGACCCCGGAAGCCCTGCTCGAATCGGCCATGCGCCACGTCGATCATCTCGACAAGCTGGACTTCCAGAACTTCAAGGTCAGCGTCAAGGCCTCCGACGTCTTCATGGCCGTCGCCGCCTATCGCCTGCTGGCCAGGCAGATCGAGCAACCCCTGCACCTGGGCATCACCGAGGCCGGCGGCCTGCGCTCCGGCACGGTGAAGTCGGCGGTGGGGCTGGGCATGCTCCTGGCCGAGGGAATCGGCGACACCATCCGGATTTCCCTGGCTGCCGATCCGGTCGAGGAGATCAAGGTCGGTTTCGACATCCTCAAGTCCCTGCACCTGCGCTCCCGTGGCATCAACTTCATCGCCTGCCCGAGCTGTTCGCGGCAGAACTTCGACGTGGTGAAGACCATGAACGAGCTGGAAGGGCGCCTGGAGGACCTGCTGGTGCCGATGGACGTGGCCGTGATCGGTTGCGTCGTCAACGGTCCGGGCGAAGCCAAGGAGGCCCATGTCGGCCTCACCGGCGGCACTCCGAACCTGGTGTATATCGACGGCAAGCCGTCGCAGAAACTGACCAACGACAACCTGGTGGACGAGCTGGAACGGCTGATCCGCCAGAAGGCGGCCGAGAAGGCCGAGGCCGATGCGAGCCTGATCGCCCGCGGCTGA
Protein Properties
Protein Residues: 371
Protein Molecular Weight: 40.1 kDa
Protein Theoretical pI: 6.41
Hydropathicity (GRAVY score): -0.049
Charge at pH 7 (predicted): -2.45
Protein Sequence: 
>PA3803
MSIHSASPIIRRKSRKIWVGNVPVGGDAPIAVQSMTNTETCDVAATVAQIRRLEDAGADIVRVSVPDMDAAEAFGKIKQQVNVPLVADIHFDYRIALRVAELGVDCLRINPGNIGREDRVKAVVDAARERNIPIRIGVNAGSLEKDLQKKYGEPTPEALLESAMRHVDHLDKLDFQNFKVSVKASDVFMAVAAYRLLARQIEQPLHLGITEAGGLRSGTVKSAVGLGMLLAEGIGDTIRISLAADPVEEIKVGFDILKSLHLRSRGINFIACPSCSRQNFDVVKTMNELEGRLEDLLVPMDVAVIGCVVNGPGEAKEAHVGLTGGTPNLVYIDGKPSQKLTNDNLVDELERLIRQKAAEKAEADASLIARG
References
External Links:
Resource Link
Genome ID: PA3803
Entrez Gene ID: 879053
NCBI Protein ID: 15598998
General Reference: PaperBLAST - Find papers about PA3803 and its homologs