Lipid-A-disaccharide synthase (PA3643)

Identification
Name: Lipid-A-disaccharide synthase
Synonyms: Not Available
Gene Name: lpxB
Enzyme Class:
Biological Properties
General Function: Involved in lipid-A-disaccharide synthase activity
Specific Function: Condensation of UDP-2,3-diacylglucosamine and 2,3- diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell
Cellular Location: Not Available
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
2,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine ↔ Hydrogen ion + 2,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + Uridine 5'-diphosphate
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb
UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine + 2,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate ↔ Uridine 5'-diphosphate + 2,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate
ReactionCard
Thumb+Thumb → Thumb+Thumb+Thumb
2,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine → Hydrogen ion + 2,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + Uridine 5'-diphosphate
ReactionCard
SMPDB Reactions:
Thumb+Thumb → Thumb+Thumb+(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate)
2,3-bis[(3R)-3-hydroxymyristoyl]-α-D-glucosaminyl 1-phosphate + UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamine → Uridine 5'-diphosphate + Hydrogen ion + (2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl)-(1->6)-(2-N,3-O-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl phosphate)
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb+Thumb ↔ Thumb+Thumb+Thumb
2,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine ↔ Hydrogen ion + 2,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + Uridine 5'-diphosphate
ReactionCard
Thumb+Thumb → Thumb+Thumb+Thumb
2,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate + UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine → Hydrogen ion + 2,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphate + Uridine 5'-diphosphate
ReactionCard
Complex Reactions:
Thumb+Thumb → Thumb+Thumb
UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphate → Uridine 5'-diphosphate + 2,3-bis(3-Hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0009052,3,2',3'-Tetrakis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-glucosamine 1-phosphateMetaboCard
PAMDB0036302,3-bis((3R)-3-hydroxymyristoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
PAMDB0009032,3-Bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
PAMDB0034832,3-bis(3-Hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphateMetaboCard
PAMDB0063342,3-bis[(3R)-3-hydroxymyristoyl]-α-D-glucosaminyl 1-phosphateMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB001047UDP-2,3-Bis(3-hydroxytetradecanoyl)glucosamineMetaboCard
PAMDB006333UDP-2-N,3-O-bis[(3R)-3-hydroxytetradecanoyl]-α-D-glucosamineMetaboCard
PAMDB000123Uridine 5'-diphosphateMetaboCard
GO Classification:
Function
catalytic activity
lipid-A-disaccharide synthase activity
transferase activity
transferase activity, transferring glycosyl groups
UDP-glycosyltransferase activity
Process
lipid A biosynthetic process
metabolic process
organophosphate metabolic process
phospholipid biosynthetic process
phospholipid metabolic process
Gene Properties
Locus tag: PA3643
Strand: -
Entrez Gene ID: 880521
Accession: NP_252333.1
GI: 15598839
Sequence start: 4081045
Sequence End: 4082181
Sequence Length: 1136
Gene Sequence: 
>PA3643
ATGGCTGACGGATTACGCGTAGCCCTGGTCGCCGGCGAGGCCTCCGGCGACATCCTCGGGTCCGGACTGATGCAGGCGCTGCGGGCGCGGCACCCGGATATCGAGTTCATCGGCGTCGGTGGGCCGCGCATGGAGGCCGAAGGGCTGTCCAGCTATTTCCCCATGGAGCGCCTGTCGGTGATGGGCCTGGTAGAAGTGCTGGGCAGGCTGCCGGAGTTGTTGCGACGGCGCAAGCGCCTGATCCGCACGCTCATCGAGGCCCGGCCGGATGTGATGATCGGCATCGACGCTCCCGACTTCACCCTCGGCGTCGAACACAAGCTGCGCCAGGCTGGCCTGCGCACCGTGCATTACGTCAGCCCTTCGGTGTGGGCCTGGCGGCAGAAGCGGGTGCTGAAGATTCGCGAAGCCTGCGACCTGATGCTGGCATTGTTCCCCTTCGAAGCACGTTTCTACGAAGAGCATGGGGTGCCCGTGCGTTTCGTCGGCCACCCCCTGGCCAACACCATTCCCCTGCAGGCGGATCGCGCTGCGGCCCGCGCGCGGCTCGGATTGCCGGCGGACGGACAGGTGCTGGCGCTGATGCCGGGCAGTCGCGGCGGCGAGGTCGGCAAGCTCGGCGCCTTGTTCCTCGACACCGCCCAGCGCCTGCTCGTCGAACGGCCGGGCCTGCGTTTCGTGCTGCCCTGCGCCAGCGCTGCACGGCGCGAACAGATCGAGCAGATGCTCCAGGGCCGCGAGCCTCTGCCGCTGACGCTGCTCGACGGCGCTTCCCATGAAGCGCTTGCCGCCTGCGATGCGGTGCTGATCGCCTCCGGTACCGCGACCCTCGAGGCGCTGCTCTACAAGCGGCCCATGGTGGTGGCTTATCGGGTCGCCGGGCTGACGTACCGGATTCTCAAGCGCCTGGTGAAAAGCCCTTACATCTCCCTGCCGAACCTGTTGGCCGGACGCCTGTTGGTGCCGGAACTGATCCAGGACGCCGCCACCCCGCAAGCGCTAGCCGCAACCCTCTCGCCGCTGCTGGACGACGGCTCGCAGCAGGTGGAATTCTTCGATGCCATCCATCGGGCCTTGCGCCAGGATGCTTCGGCACAGGCGGCGGAAGCCGTCCTGCAACTGGTGGAGCGCCGCTGA
Protein Properties
Protein Residues: 378
Protein Molecular Weight: 41.3 kDa
Protein Theoretical pI: 9.39
Hydropathicity (GRAVY score): 0.13
Charge at pH 7 (predicted): 6.6
Protein Sequence: 
>PA3643
MADGLRVALVAGEASGDILGSGLMQALRARHPDIEFIGVGGPRMEAEGLSSYFPMERLSVMGLVEVLGRLPELLRRRKRLIRTLIEARPDVMIGIDAPDFTLGVEHKLRQAGLRTVHYVSPSVWAWRQKRVLKIREACDLMLALFPFEARFYEEHGVPVRFVGHPLANTIPLQADRAAARARLGLPADGQVLALMPGSRGGEVGKLGALFLDTAQRLLVERPGLRFVLPCASAARREQIEQMLQGREPLPLTLLDGASHEALAACDAVLIASGTATLEALLYKRPMVVAYRVAGLTYRILKRLVKSPYISLPNLLAGRLLVPELIQDAATPQALAATLSPLLDDGSQQVEFFDAIHRALRQDASAQAAEAVLQLVERR
References
External Links:
Resource Link
Genome ID: PA3643
Entrez Gene ID: 880521
NCBI Protein ID: 15598839
General Reference: PaperBLAST - Find papers about PA3643 and its homologs