Enolase (PA3635)

Identification
Name: Enolase
Synonyms:
  • 2-phospho-D-glycerate hydro-lyase
  • 2-phosphoglycerate dehydratase
Gene Name: eno
Enzyme Class:
Biological Properties
General Function: Involved in magnesium ion binding
Specific Function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. It is also a component of the RNA degradosome, a multi-enzyme complex involved in RNA processing and messenger RNA degradation. Its interaction with RNase E is important for the turnover of mRNA, in particular on transcripts encoding enzymes of energy-generating metabolic routes. Its presence in the degradosome is required for the response to excess phosphosugar. May play a regulatory role in the degradation of specific RNAs, such as ptsG mRNA, therefore linking cellular metabolic status with post-translational gene regulation
Cellular Location: Cytoplasm, cytoskeleton. Secreted. Cell surface.
KEGG Pathways:
KEGG Reactions:
Thumb ↔ Thumb+Thumb
2-Phospho-D-glyceric acid ↔ Water + Phosphoenolpyruvic acid
ReactionCard
SMPDB Reactions:
Thumb → Thumb+Thumb
2-Phospho-D-glyceric acid → Phosphoenolpyruvic acid + Water
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Thumb → Thumb+2-Phosphoglyceric acid+Thumb
Phosphoenolpyruvic acid → Water + 2-Phosphoglyceric acid + 2-Phosphoglyceric acid
ReactionCard
2-Phosphoglyceric acid+Thumb ↔ Thumb+Thumb
2-Phosphoglyceric acid + 2-Phosphoglyceric acid ↔ Water + Phosphoenolpyruvic acid
ReactionCard
PseudoCyc/BioCyc Reactions:
Thumb ↔ Thumb+Thumb
2-Phospho-D-glyceric acid ↔ Water + Phosphoenolpyruvic acid
ReactionCard
Complex Reactions:
Thumb → Thumb+Thumb
2-Phospho-D-glyceric acid → Phosphoenolpyruvic acid + Water
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0006262-Phospho-D-glyceric acidMetaboCard
PAMDB0018142-Phosphoglyceric acidMetaboCard
PAMDB000111Phosphoenolpyruvic acidMetaboCard
PAMDB000142WaterMetaboCard
GO Classification:
Component
macromolecular complex
phosphopyruvate hydratase complex
protein complex
Function
binding
carbon-oxygen lyase activity
catalytic activity
cation binding
hydro-lyase activity
ion binding
lyase activity
magnesium ion binding
metal ion binding
phosphopyruvate hydratase activity
Process
alcohol metabolic process
glucose catabolic process
glucose metabolic process
glycolysis
hexose metabolic process
metabolic process
monosaccharide metabolic process
small molecule metabolic process
Gene Properties
Locus tag: PA3635
Strand: -
Entrez Gene ID: 880453
Accession: NP_252325.1
GI: 15598831
Sequence start: 4068677
Sequence End: 4069966
Sequence Length: 1289
Gene Sequence: 
>PA3635
ATGGCAAAGATCGTCGACATCAAGGGCCGTGAGGTCCTGGACTCCCGCGGCAACCCTACCGTTGAAGCGGACGTGATCCTGGACAACGGCATCGTCGGCAGCGCCTGCGCGCCTTCCGGTGCTTCCACCGGTTCCCGCGAGGCCCTCGAGCTGCGCGATGGCGACAAGAGCCGTTACCTGGGCAAGGGCGTGCTGAAAGCCGTGGCCAACATCAACGGCCCGATCCGCGACCTGCTGCTGGGCAAGGACGCGGCCGACCAGAAAGCCCTCGACCACGCGATGATCGAGCTGGACGGCACCGAGAACAAGGCCAAGCTGGGCGCCAACGCGATCCTCGCGGTGTCCCTGGCTGCCGCCAAGGCCGCCGCACAGGCCAAGGGCGTACCGCTGTACGCGCACATCGCCGACCTCAACGGCACTCCCGGCCAGTACTCCATGCCGGTGCCGATGATGAACATCATCAACGGCGGCGAGCATGCGGACAACAACGTCGATATCCAGGAATTCATGGTCCAGCCGGTCGGCGCGAAGAACTTCGCCGAGGCGCTGCGCATGGGCGCCGAGATCTTCCATCACCTCAAGGCCGTGCTGAAGGCCCGTGGCCTGAACACCGCCGTCGGTGACGAAGGCGGCTTCGCGCCGAACCTGTCGTCCAACGAAGACGCCCTGGCCGCCATCGCCGAGGCCGTCGAGAAGGCCGGCTACAAGCTGGGCGACGACGTGACCCTGGCCCTGGACTGCGCCTCCAGCGAGTTCTTCAAGGACGGCAAGTACGACCTGGAAGGCGAAGGCAAGGTATTCGACGCCGCCGGTTTCGCCGACTACCTGGCCGGCCTGACCCAGCGCTACCCGATCATCTCCATCGAGGACGGCATGGACGAGTCCGACTGGGCCGGCTGGAAAGGCCTGACCGACAAGATCGGCGCCAAGGTCCAACTGGTCGGCGACGACCTGTTCGTGACCAACACCAAGATCCTCAAGGAAGGCATCGAGAAGGGCATCGGCAATTCGATCCTGATCAAGTTCAACCAGATCGGTTCGCTCACCGAGACCCTGGAGGCCATCCAGATGGCCAAGGCCGCCGGCTATACCGCGGTGATCTCGCACCGCTCCGGCGAAACCGAGGACTCGACCATCGCCGACCTGGCCGTGGGTACCGCCGCCGGTCAGATCAAGACCGGTTCGCTGTGCCGCTCCGACCGCGTGTCCAAGTACAACCAGTTGCTGCGCATCGAAGAGCAACTGGGCGCCAAGGCGCCGTACCGTGGTCGCGCGGAATTCCGCGGCTGA
Protein Properties
Protein Residues: 429
Protein Molecular Weight: 45.2 kDa
Protein Theoretical pI: 4.81
Hydropathicity (GRAVY score): -0.142
Charge at pH 7 (predicted): -12.63
Protein Sequence: 
>PA3635
MAKIVDIKGREVLDSRGNPTVEADVILDNGIVGSACAPSGASTGSREALELRDGDKSRYLGKGVLKAVANINGPIRDLLLGKDAADQKALDHAMIELDGTENKAKLGANAILAVSLAAAKAAAQAKGVPLYAHIADLNGTPGQYSMPVPMMNIINGGEHADNNVDIQEFMVQPVGAKNFAEALRMGAEIFHHLKAVLKARGLNTAVGDEGGFAPNLSSNEDALAAIAEAVEKAGYKLGDDVTLALDCASSEFFKDGKYDLEGEGKVFDAAGFADYLAGLTQRYPIISIEDGMDESDWAGWKGLTDKIGAKVQLVGDDLFVTNTKILKEGIEKGIGNSILIKFNQIGSLTETLEAIQMAKAAGYTAVISHRSGETEDSTIADLAVGTAAGQIKTGSLCRSDRVSKYNQLLRIEEQLGAKAPYRGRAEFRG
References
External Links:
Resource Link
Genome ID: PA3635
Entrez Gene ID: 880453
NCBI Protein ID: 15598831
General Reference: PaperBLAST - Find papers about PA3635 and its homologs