NADPH-dependent 7-cyano-7-deazaguanine reductase (PA2806)

Identification
Name: NADPH-dependent 7-cyano-7-deazaguanine reductase
Synonyms:
  • 7-cyano-7-carbaguanine reductase
  • NADPH-dependent nitrile oxidoreductase
  • PreQ(0) reductase
Gene Name: queF
Enzyme Class:
Biological Properties
General Function: Involved in oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
Specific Function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)
Cellular Location: Cytoplasm (Probable)
KEGG Pathways:
KEGG Reactions:
Thumb+Thumb ↔ Thumb+Thumb+2 Thumb
7-Aminomethyl-7-carbaguanine + NADP ↔ 7-Cyano-7-carbaguanine + NADPH + 2 Hydrogen ion
ReactionCard
SMPDB Reactions:
Thumb+3 Thumb+2 NADPH+2 Thumb → 2 Thumb+Thumb
7-Cyano-7-carbaguanine + 3 Hydrogen ion + 2 NADPH + 2 NADPH → 2 NADP + 7-Aminomethyl-7-carbaguanine
ReactionCard
Thumb+3 Thumb+2 NADPH+2 Thumb → Queuine+2 Thumb+Thumb
7-Cyano-7-carbaguanine + 3 Hydrogen ion + 2 NADPH + 2 NADPH → Queuine + 2 NADP + Queuine
ReactionCard
PseudoCyc/BioCyc Reactions:
3 Thumb+2 Thumb+Thumb → 2 Thumb+Thumb
3 Hydrogen ion + 2 NADPH + 7-Cyano-7-carbaguanine → 2 NADP + Queuine
ReactionCard
Thumb+2 Thumb → Thumb+2 Thumb
7-Aminomethyl-7-carbaguanine + 2 NADP → 7-Cyano-7-carbaguanine + 2 NADPH
ReactionCard
Thumb+Thumb ↔ Thumb+Thumb+2 Thumb
7-Aminomethyl-7-carbaguanine + NADP ↔ 7-Cyano-7-carbaguanine + NADPH + 2 Hydrogen ion
ReactionCard
Thumb+3 Thumb+2 NADPH+2 Thumb → 2 Thumb+Thumb
7-Cyano-7-carbaguanine + 3 Hydrogen ion + 2 NADPH + 2 NADPH → 2 NADP + 7-Aminomethyl-7-carbaguanine
ReactionCard
Thumb+3 Thumb+2 NADPH+2 Thumb → Queuine+2 Thumb+Thumb
7-Cyano-7-carbaguanine + 3 Hydrogen ion + 2 NADPH + 2 NADPH → Queuine + 2 NADP + Queuine
ReactionCard
PAMDB120135+PAMDB110541 → PAMDB001132+PAMDB110539+PAMDB001633
preQ1 + NADP+ → 7-Cyano-7-carbaguanine + NADPH + Hydrogen ion
ReactionCard
Complex Reactions:
3 Thumb+2 Thumb+Thumb → 2 Thumb+Thumb
3 Hydrogen ion + 2 NADPH + 7-Cyano-7-carbaguanine → 2 NADP + Queuine
ReactionCard
Thumb+2 Thumb → Thumb+2 Thumb
7-Aminomethyl-7-carbaguanine + 2 NADP → 7-Cyano-7-carbaguanine + 2 NADPH
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB0018517-Aminomethyl-7-carbaguanineMetaboCard
PAMDB0011327-Cyano-7-carbaguanineMetaboCard
PAMDB001633Hydrogen ionMetaboCard
PAMDB000089NADPMetaboCard
PAMDB000615NADPHMetaboCard
PAMDB000403QueuineMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
catalytic activity
oxidoreductase activity
oxidoreductase activity, acting on other nitrogenous compounds as donors
oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor
Process
cellular macromolecule metabolic process
macromolecule metabolic process
metabolic process
ncRNA metabolic process
oxidation reduction
queuosine biosynthetic process
RNA metabolic process
tRNA metabolic process
tRNA modification
tRNA processing
Gene Properties
Locus tag: PA2806
Strand: -
Entrez Gene ID: 880562
Accession: NP_251496.1
GI: 15598002
Sequence start: 3160651
Sequence End: 3161481
Sequence Length: 830
Gene Sequence: 
>PA2806
ATGCAGCATCCCGCCGAACATTCGCCGCTGGGCAAGACCAGCGAATACGTCTCCAGCTACACGCCGTCGCTGCTGTTTCCCATTTCACGCACGGCGAAATGGGCCGAGCTTGGCCTGAGCGCCGAGACCCTGCCTTATCGCGGCGTGGATATCTGGAACTGCTACGAGCTGTCCTGGCTGACCCCGGCCGGCAAGCCGGTGGTGGCGATCGGCGAGTTCTCGATCCCGGCGGACTCGCCGAACATCATCGAGTCGAAGTCGTTCAAGCTTTACCTCAATTCCCTCAACCAGTCCGCCTTCGATAGTCGCGAGGCGCTTCGCGCGGTATTGCAGAAAGACCTCTCGGCCGCTGTCGGGGCGCCCGTGGGCGTGCGCCTGCGCAGCCTCGACGAAGTTGCCGAGGAGGGCATCGGGCGTCTGCCGGGGCGCTGCATCGACGAGCTGGACATCGCCGTCGACGGCTACGAGCAGCCGCGCCCGGAACTGCTGCGCTGCGACGCCGGGCGGATCGTCGAGGAGCAGCTCTACAGCCACCTGCTCAAGTCCAACTGCCCGGTCACCGGCCAGCCCGACTGGGGCACCCTGGTGGTCGACTACCGGGGCCCGGCGCTGGACCCGGCCAGCCTGCTGGCCTACCTGGTTTCCTTCCGCCAGCACCAGGACTTCCACGAACAGTGCGTCGAACGCATCTTCCTCGACCTGCAGCGCCTGTTGCAGCCGCAGGCGCTCAGCGTCTACGCGCGCTACGTACGCCGTGGCGGCCTGGATATCAATCCCTACCGCAGCCTGGCGGAGGTCGCACCGGACAACCGACGCCTGGTGCGCCAGTAA
Protein Properties
Protein Residues: 276
Protein Molecular Weight: 30.8 kDa
Protein Theoretical pI: 5.41
Hydropathicity (GRAVY score): -0.239
Charge at pH 7 (predicted): -4.95
Protein Sequence: 
>PA2806
MQHPAEHSPLGKTSEYVSSYTPSLLFPISRTAKWAELGLSAETLPYRGVDIWNCYELSWLTPAGKPVVAIGEFSIPADSPNIIESKSFKLYLNSLNQSAFDSREALRAVLQKDLSAAVGAPVGVRLRSLDEVAEEGIGRLPGRCIDELDIAVDGYEQPRPELLRCDAGRIVEEQLYSHLLKSNCPVTGQPDWGTLVVDYRGPALDPASLLAYLVSFRQHQDFHEQCVERIFLDLQRLLQPQALSVYARYVRRGGLDINPYRSLAEVAPDNRRLVRQ
References
External Links:
Resource Link
Genome ID: PA2806
Entrez Gene ID: 880562
NCBI Protein ID: 15598002
General Reference: PaperBLAST - Find papers about PA2806 and its homologs