P. aeruginosa Metabolome Database Protein PA4302

Identification

Name:

tRNA-specific adenosine deaminase

Synonyms:

Not Available

Gene Name:

tadA

Enzyme Class:

3.5.4.33

Biological Properties

General Function:

Energy production and conversion

Specific Function:

Deaminates adenosine-34 to inosine in tRNA-Arg2. Mutation in this protein makes E.coli resistant to the toxic proteins encoded by the gef gene family. Essential for cell viability

Cellular Location:

Cytoplasmic

KEGG Pathways:

Not Available

KEGG Reactions:

→

Water + Adenosine → Ammonia + Inosine
ReactionCard

↔

Water ↔ Ammonia
ReactionCard

SMPDB Reactions:

Not Available

PseudoCyc/BioCyc Reactions:

→

Water + Adenosine → Ammonia + Inosine
ReactionCard

Complex Reactions:

→

Adenosine + Water → Inosine + Ammonia
ReactionCard

Transports:

Not Available

Metabolites:

PAMDB ID	Name	View
PAMDB000016	Adenosine	MetaboCard
PAMDB000017	Ammonia	MetaboCard
PAMDB000082	Inosine	MetaboCard
PAMDB000142	Water	MetaboCard

GO Classification:

Function
binding
catalytic activity
cation binding
hydrolase activity
ion binding
metal ion binding
transition metal ion binding
zinc ion binding

Gene Properties

Locus tag:

PA4302

Strand:

Entrez Gene ID:

881597

Accession:

NP_252992.1

GI:

15599498

Sequence start:

4825923

Sequence End:

4827188

Sequence Length:

1265

Gene Sequence:

>PA4302
ATGAGCACGGGATTCGGCGCGCGTCCTGACGGCGGTGCCTTCCACTCCCGCCAGGAGCAGGACATCCAGGCGCTGAAGCTGCGCCTGCACCGCTACATCATCGACGAGATCGACGAAGACGGCATGAACCTGCTGGAGGGCGCGCGCTCCGCCGTGGTCCAGTACGTCTCGGAAAAGGTCTGCGAATACGGCTCGCGCCATCAACTGGCGATCTCCCGCTACGAACTGGACCGCCTGGCCGAAGAGGTGGTCGACGAACTCACCGGCTTCGGCCCGCTGGAGATCCTCCTGCGCGATCCCGGCGTCTCGGAAATCCTCGTCAACGGGCCGGGCCGGGTGTTCGTCGAGCGGGAAGGGCGGCTCTACCAGAGCGACCTGCGCTTCATCGACGACCACCATGTGCTGCGGGTGATCCAGCGTATCCTCGCGCCCCTCGGCCGACGCCTGGACGAGTCCAGCCCGATGGTCGACGCGCGCCTGCCGGACGGCAGCCGGGTCAACGCGATCATTCCCCCGGTGGCGCTGGACGGGCCGTGCATCTCGATCCGCAAGTTCAGCCAGGAGCTGCTGCGCAGTGCCGACCTGCTGGCCTACCAGAGCGTCGACGAAGCCTTGCTCGAGTTCCTCCGCCAGGCGGTCTCGCGCCGCTGCAACATCCTCATCAGCGGCGGCACCGGCACCGGCAAGACCACCTTGCTCAACGTCATCAGCGGCTTCATCGACGAACGCGAGCGGATCGTCACCATCGAGGACACCGCCGAGCTGCAACTCGGCCACGACCACGTGGTGCGCCTGGAGACCCGTCCGCCGAACGCCGAGGGCTACGGCGAGGTGACCGCCCGCGACCTGATCCGCAACGCCCTGCGGATGCGCCCGGACCGGATCATCCTCGGCGAGATCCGTGGCGTCGAGGTGCTCGACGTGCTGCAGGCGATGAACACCGGCCACGACGGCTCGATGAGCACGGTGCACGCCAACTCGGCGATGGATTCGCTGCTGCGCATGGAGATGCTGGTCGGCCTGACCGGCAACAGGCTGCCGGAGCAGACCTTGCGCCTGATGATTTGCGCCGCGCTGGACGTGGTGGTGCAGATCACCCGCCTGGCCAGCGGTCGGCGTTGCATCAGCGAAGTGCTGGAGGTGCTGGAGGTGCGCGACGGTGTGTACGTCACCAATACGCTGTTCAGTCTCGACCGGCGCGGTAGCGGGCAGTTCGTCCGCCAGGCCGCGCCGGTCGGGCAGAAATTCCGCCAGGCGCTGCTGTGA

Protein Properties

Protein Residues:

421

Protein Molecular Weight:

46.8 kDa

Protein Theoretical pI:

5.52

Hydropathicity (GRAVY score):

-0.115

Charge at pH 7 (predicted):

-7.96

Protein Sequence:

>PA4302
MSTGFGARPDGGAFHSRQEQDIQALKLRLHRYIIDEIDEDGMNLLEGARSAVVQYVSEKVCEYGSRHQLAISRYELDRLAEEVVDELTGFGPLEILLRDPGVSEILVNGPGRVFVEREGRLYQSDLRFIDDHHVLRVIQRILAPLGRRLDESSPMVDARLPDGSRVNAIIPPVALDGPCISIRKFSQELLRSADLLAYQSVDEALLEFLRQAVSRRCNILISGGTGTGKTTLLNVISGFIDERERIVTIEDTAELQLGHDHVVRLETRPPNAEGYGEVTARDLIRNALRMRPDRIILGEIRGVEVLDVLQAMNTGHDGSMSTVHANSAMDSLLRMEMLVGLTGNRLPEQTLRLMICAALDVVVQITRLASGRRCISEVLEVLEVRDGVYVTNTLFSLDRRGSGQFVRQAAPVGQKFRQALL

References

External Links:

Resource	Link
Genome ID:	PA4302
Entrez Gene ID:	881597
NCBI Protein ID:	15599498

General Reference:

PaperBLAST - Find papers about PA4302 and its homologs

tRNA-specific adenosine deaminase (PA4302)