3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (PA1610)

Identification
Name: 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
Synonyms:
  • Beta-hydroxydecanoyl thioester dehydrase
Gene Name: fabA
Enzyme Class:
Biological Properties
General Function: Involved in catalytic activity
Specific Function: Necessary for the introduction of cis unsaturation into fatty acids. Catalyzed the dehydration of (3R)-3-hydroxydecanoyl- ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)- decenoyl-ACP
Cellular Location: Cytoplasm
KEGG Pathways:
KEGG Reactions:
(3R)-3-Hydroxydecanoyl-[acyl-carrier protein] ↔ trans-Dec-2-enoyl-[acp]+Thumb
(3R)-3-Hydroxydecanoyl-[acyl-carrier protein] ↔ trans-Dec-2-enoyl-[acp] + Water
ReactionCard
Thumb ↔ trans-2,3-Dehydroacyl-[acyl-carrier protein]+Thumb
(3R)-3-Hydroxyacyl-[acyl-carrier protein] ↔ trans-2,3-Dehydroacyl-[acyl-carrier protein] + Water
ReactionCard
SMPDB Reactions:
(3R)-3-hydroxyacyl-[acyl-carrier protein] → Thumb+ trans-2-enoyl-[acyl-carrier protein]
(3R)-3-hydroxyacyl-[acyl-carrier protein] → Water + trans-2-enoyl-[acyl-carrier protein]
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an (R)-3-hydroxydecanoyl-[acp]? → Thumb+a trans-?2-decenoyl-[acp]?
an (R)-3-hydroxydecanoyl-[acp]? → Water + a trans-?2-decenoyl-[acp]?
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a trans-?2-decenoyl-[acp]? → cis-?3-decenoyl-ACP
a trans-?2-decenoyl-[acp]? → cis-?3-decenoyl-ACP
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PseudoCyc/BioCyc Reactions:
(R)-3-hydroxy-cis-dodec-5-enoyl-[acyl-carrier protein] → Thumb+trans-3-cis-5-dodecenoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-dodec-5-enoyl-[acyl-carrier protein] → Water + trans-3-cis-5-dodecenoyl-[acyl-carrier protein]
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(R)-3-hydroxy-cis-palm-9-eoyl-[acyl-carrier protein] → Thumb+trans-3-cis-9-palmitoleoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-palm-9-eoyl-[acyl-carrier protein] → Water + trans-3-cis-9-palmitoleoyl-[acyl-carrier protein]
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(R)-3-Hydroxydodecanoyl-[acyl-carrier protein] → Thumb+trans-Dodec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxydodecanoyl-[acyl-carrier protein] → Water + trans-Dodec-2-enoyl-[acyl-carrier protein]
ReactionCard
(3R)-3-Hydroxyacyl-[acyl-carrier protein] → But-2-enoyl-[acyl-carrier protein]+Thumb
(3R)-3-Hydroxyacyl-[acyl-carrier protein] → But-2-enoyl-[acyl-carrier protein] + Water
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(R)-3-hydroxy-cis-myristol-7-eoyl-[acyl-carrier protein] → Thumb+trans-3-cis-7-myristoleoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-myristol-7-eoyl-[acyl-carrier protein] → Water + trans-3-cis-7-myristoleoyl-[acyl-carrier protein]
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(R)-3-hydroxy-cis-vacc-11-enoyl-[acyl-carrier protein] → Thumb+trans-3-cis-11-vacceoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-vacc-11-enoyl-[acyl-carrier protein] → Water + trans-3-cis-11-vacceoyl-[acyl-carrier protein]
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(R)-3-Hydroxydecanoyl-[acyl-carrier protein] → Thumb+trans-Dec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxydecanoyl-[acyl-carrier protein] → Water + trans-Dec-2-enoyl-[acyl-carrier protein]
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(R)-3-Hydroxyhexanoyl-[acyl-carrier protein] → Thumb+trans-Hex-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxyhexanoyl-[acyl-carrier protein] → Water + trans-Hex-2-enoyl-[acyl-carrier protein]
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(R)-3-Hydroxyoctadecanoyl-[acyl-carrier protein] → Thumb+trans-octadec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxyoctadecanoyl-[acyl-carrier protein] → Water + trans-octadec-2-enoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxyoctanoyl-[acyl-carrier protein] → Thumb+trans-Oct-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxyoctanoyl-[acyl-carrier protein] → Water + trans-Oct-2-enoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] → Thumb+trans-Tetradec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] → Water + trans-Tetradec-2-enoyl-[acyl-carrier protein]
ReactionCard
R-3-hydroxypalmitoyl-[acyl-carrier protein] → Thumb+trans-Hexadec-2-enoyl-[acyl-carrier protein]
R-3-hydroxypalmitoyl-[acyl-carrier protein] → Water + trans-Hexadec-2-enoyl-[acyl-carrier protein]
ReactionCard
(3R)-3-Hydroxydecanoyl-[acyl-carrier protein] ↔ trans-Dec-2-enoyl-[acp]+Thumb
(3R)-3-Hydroxydecanoyl-[acyl-carrier protein] ↔ trans-Dec-2-enoyl-[acp] + Water
ReactionCard
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → trans-dec-2-enoyl-[acyl-carrier-protein]+Thumb
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → trans-dec-2-enoyl-[acyl-carrier-protein] + Water
ReactionCard
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → cis-dec-3-enoyl-[acyl-carrier-protein]+Thumb
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → cis-dec-3-enoyl-[acyl-carrier-protein] + Water
ReactionCard
Thumb ↔ trans-2,3-Dehydroacyl-[acyl-carrier protein]+Thumb
(3R)-3-Hydroxyacyl-[acyl-carrier protein] ↔ trans-2,3-Dehydroacyl-[acyl-carrier protein] + Water
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(3R)-3-hydroxyacyl-[acyl-carrier protein] → Thumb+ trans-2-enoyl-[acyl-carrier protein]
(3R)-3-hydroxyacyl-[acyl-carrier protein] → Water + trans-2-enoyl-[acyl-carrier protein]
ReactionCard
an (R)-3-hydroxydecanoyl-[acp]? → Thumb+a trans-?2-decenoyl-[acp]?
an (R)-3-hydroxydecanoyl-[acp]? → Water + a trans-?2-decenoyl-[acp]?
ReactionCard
Thumb
a (3R)-3-hydroxyacyl-[acyl-carrier protein] → a trans-2-enoyl-[acyl-carrier protein] + H2O
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a (3R)-3-hydroxydecanoyl-[acp] → a (2E)-dec-2-enoyl-[acp] + H2O
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Complex Reactions:
(R)-3-hydroxy-cis-dodec-5-enoyl-[acyl-carrier protein] → Thumb+trans-3-cis-5-dodecenoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-dodec-5-enoyl-[acyl-carrier protein] → Water + trans-3-cis-5-dodecenoyl-[acyl-carrier protein]
ReactionCard
(R)-3-hydroxy-cis-palm-9-eoyl-[acyl-carrier protein] → Thumb+trans-3-cis-9-palmitoleoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-palm-9-eoyl-[acyl-carrier protein] → Water + trans-3-cis-9-palmitoleoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxydodecanoyl-[acyl-carrier protein] → Thumb+trans-Dodec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxydodecanoyl-[acyl-carrier protein] → Water + trans-Dodec-2-enoyl-[acyl-carrier protein]
ReactionCard
(3R)-3-Hydroxyacyl-[acyl-carrier protein] → But-2-enoyl-[acyl-carrier protein]+Thumb
(3R)-3-Hydroxyacyl-[acyl-carrier protein] → But-2-enoyl-[acyl-carrier protein] + Water
ReactionCard
(R)-3-hydroxy-cis-myristol-7-eoyl-[acyl-carrier protein] → Thumb+trans-3-cis-7-myristoleoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-myristol-7-eoyl-[acyl-carrier protein] → Water + trans-3-cis-7-myristoleoyl-[acyl-carrier protein]
ReactionCard
(R)-3-hydroxy-cis-vacc-11-enoyl-[acyl-carrier protein] → Thumb+trans-3-cis-11-vacceoyl-[acyl-carrier protein]
(R)-3-hydroxy-cis-vacc-11-enoyl-[acyl-carrier protein] → Water + trans-3-cis-11-vacceoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxydecanoyl-[acyl-carrier protein] → Thumb+trans-Dec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxydecanoyl-[acyl-carrier protein] → Water + trans-Dec-2-enoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxyhexanoyl-[acyl-carrier protein] → Thumb+trans-Hex-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxyhexanoyl-[acyl-carrier protein] → Water + trans-Hex-2-enoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxyoctadecanoyl-[acyl-carrier protein] → Thumb+trans-octadec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxyoctadecanoyl-[acyl-carrier protein] → Water + trans-octadec-2-enoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxyoctanoyl-[acyl-carrier protein] → Thumb+trans-Oct-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxyoctanoyl-[acyl-carrier protein] → Water + trans-Oct-2-enoyl-[acyl-carrier protein]
ReactionCard
(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] → Thumb+trans-Tetradec-2-enoyl-[acyl-carrier protein]
(R)-3-Hydroxytetradecanoyl-[acyl-carrier protein] → Water + trans-Tetradec-2-enoyl-[acyl-carrier protein]
ReactionCard
R-3-hydroxypalmitoyl-[acyl-carrier protein] → Thumb+trans-Hexadec-2-enoyl-[acyl-carrier protein]
R-3-hydroxypalmitoyl-[acyl-carrier protein] → Water + trans-Hexadec-2-enoyl-[acyl-carrier protein]
ReactionCard
trans-Dec-2-enoyl-[acyl-carrier protein] ↔ cis-dec-3-enoyl-[acyl-carrier protein] (n-C10:1)
trans-Dec-2-enoyl-[acyl-carrier protein] ↔ cis-dec-3-enoyl-[acyl-carrier protein] (n-C10:1)
ReactionCard
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → trans-dec-2-enoyl-[acyl-carrier-protein]+Thumb
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → trans-dec-2-enoyl-[acyl-carrier-protein] + Water
ReactionCard
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → cis-dec-3-enoyl-[acyl-carrier-protein]+Thumb
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] → cis-dec-3-enoyl-[acyl-carrier-protein] + Water
ReactionCard
Transports: Not Available
Metabolites:
PAMDB IDNameView
PAMDB000142WaterMetaboCard
GO Classification:
Component
cell part
cytoplasm
intracellular part
Function
3-hydroxyacyl-[acyl-carrier-protein] dehydratase activity
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity
carbon-oxygen lyase activity
catalytic activity
hydro-lyase activity
lyase activity
Process
biosynthetic process
carboxylic acid metabolic process
cellular metabolic process
fatty acid biosynthetic process
fatty acid metabolic process
metabolic process
monocarboxylic acid metabolic process
organic acid metabolic process
oxoacid metabolic process
Gene Properties
Locus tag: PA1610
Strand: -
Entrez Gene ID: 881984
Accession: NP_250301.1
GI: 15596807
Sequence start: 1752903
Sequence End: 1753418
Sequence Length: 515
Gene Sequence: 
>PA1610
ATGACCAAACAACACGCCTTCACCCGAGAAGACCTGCTGCGCTGCAGTCGCGGCGAGCTGTTCGGCCCGGGTAACGCGCAACTTCCCGCCCCCAACATGCTGATGATCGATCGCATCGTTCACATCAGCGATGTCGGCGGCAAGTATGGCAAGGGCGAGCTGGTCGCCGAGCTGGATATCAACCCGGACCTGTGGTTCTTCGCCTGTCACTTCGAAGGCGATCCGGTCATGCCCGGTTGCCTGGGCCTGGATGCCATGTGGCAGCTGGTCGGTTTCTACCTCGGCTGGCAGGGCAACCCCGGCCGCGGCCGCGCCCTAGGTTCGGGCGAAGTGAAGTTCTTCGGTCAGGTTCTGCCGACAGCGAAGAAGGTCACGTATAACATTCATATCAAGCGCACGATCAATCGTTCGCTGGTCCTGGCCATCGCCGACGGCACCGTCAGCGTCGATGGCCGCGAAATCTACAGTGCCGAAGGCCTCCGCGTCGGCCTGTTCACCTCCACTGACAGCTTCTAA
Protein Properties
Protein Residues: 171
Protein Molecular Weight: 18.7 kDa
Protein Theoretical pI: 6.93
Hydropathicity (GRAVY score): -0.023
Charge at pH 7 (predicted): -0.14
Protein Sequence: 
>PA1610
MTKQHAFTREDLLRCSRGELFGPGNAQLPAPNMLMIDRIVHISDVGGKYGKGELVAELDINPDLWFFACHFEGDPVMPGCLGLDAMWQLVGFYLGWQGNPGRGRALGSGEVKFFGQVLPTAKKVTYNIHIKRTINRSLVLAIADGTVSVDGREIYSAEGLRVGLFTSTDSF
References
External Links:
Resource Link
Genome ID: PA1610
Entrez Gene ID: 881984
NCBI Protein ID: 15596807
General Reference: PaperBLAST - Find papers about PA1610 and its homologs