D-alanyl-D-alanine endopeptidase (PA0869)

Identification
Name: D-alanyl-D-alanine endopeptidase
Synonyms:
  • DD-endopeptidase
  • Penicillin-binding protein 7
  • PBP-7
Gene Name: pbpG
Enzyme Class: Not Available
Biological Properties
General Function: Involved in serine-type D-Ala-D-Ala carboxypeptidase activity
Specific Function: Cell wall formation. May play a specialized role in remodeling the cell wall. Specifically hydrolyze the DD- diaminopimelate-alanine bonds in high-molecular-mass murein sacculi
Cellular Location: Periplasm
KEGG Pathways:
    KEGG Reactions: Not Available
    SMPDB Reactions: Not Available
    PseudoCyc/BioCyc Reactions:
    peptidoglycan D-alanyl-DAP crosslink+Thumb ↔ peptidoglycan tetrapeptide, glycan chain 2+peptidoglycan tetrapeptide, glycan chain 1
    peptidoglycan D-alanyl-DAP crosslink + Water ↔ peptidoglycan tetrapeptide, glycan chain 2 + peptidoglycan tetrapeptide, glycan chain 1
    ReactionCard
    Complex Reactions:
    Thumb+three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala) & tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain) → three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain)
    Water + three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala) & tetrapeptide corsslinked tetrapeptide (A2pm->D-ala)) (middle of chain) → three disacharide linked murein units (tetrapeptide crosslinked tetrapeptide (A2pm->D-ala), one uncrosslinked tetrapaptide) (middle of chain)
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    Thumb+two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain)
    Water + two disacharide linked murein units, pentapeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → two linked disacharide pentapeptide and tetrapeptide murein units (uncrosslinked, middle of chain)
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    Thumb+two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain) → two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain)
    Water + two disacharide linked murein units, tetrapeptide corsslinked tetrapeptide (A2pm->D-ala) (middle of chain) → two linked disacharide tetrapeptide murein units (uncrosslinked, middle of chain)
    ReactionCard
    Thumb+two disacharide linked murein units, tripeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain)
    Water + two disacharide linked murein units, tripeptide crosslinked tetrapeptide (A2pm->D-ala) (middle of chain) → two linked disacharide tetrapeptide and tripeptide murein units (uncrosslinked, middle of chain)
    ReactionCard
    Transports: Not Available
    Metabolites:
    PAMDB IDNameView
    PAMDB000142WaterMetaboCard
    GO Classification:
    Function
    carboxypeptidase activity
    catalytic activity
    exopeptidase activity
    hydrolase activity
    peptidase activity
    peptidase activity, acting on L-amino acid peptides
    serine-type carboxypeptidase activity
    serine-type D-Ala-D-Ala carboxypeptidase activity
    Process
    macromolecule metabolic process
    metabolic process
    protein metabolic process
    proteolysis
    Gene Properties
    Locus tag: PA0869
    Strand: -
    Entrez Gene ID: 877694
    Accession: NP_249560.1
    GI: 15596066
    Sequence start: 949716
    Sequence End: 950648
    Sequence Length: 932
    Gene Sequence: 
    >PA0869
GTGAGAAACCGTCTCCTGTCACTGGTCACCCTGTTCCTGTCCCTCAGCGTTGCCACCGCGGTATCCGCCTCCCCGCCGCCCAAGGCCGGGCTGCCGCTGCAACAGGAGCTGGCGTCGGGCAGCGCGCTGGTTACCGACCAGAACACCGGCAAGGTACTCTACTCGCGCAACCCCGACCTGGTGGTGCCGATCGCCTCAATCACCAAGCTGATGACCGCCATGGTCGTGCTCGACTCGAAACTGCCGCTGGACGAGGTACTGCCGATCGCCATCAGCGAGACCAGCGAAATGCGCGGGGTGTTTTCCCGGGTGCGGGTCGGCAGCCAGATCAGCCGCCGGGACATGCTCCTGCTCGCCCTGATGTCCTCGGAGAACCGCGCCGCCGCCAGCCTCGCGCAGAACTATCCGGGCGGCAAGAACGCCTTCGTCAAGGCGATGAACGCCAAGGCCCACGCCCTGGGAATGAAGAACACCCGCTACGTCGAGCCGACCGGGCTGTCGCTGCACAACGTCTCCACCGCTCGCGACCTCACCCGCCTGCTGGTGGCCAGCCGGCAATATCCGCTGCTCAGTCAGTGGAGCACCACTCCGGAGAAGACCGTGGCCTTCCGCCACCCCAACTACACCCTGGGCTTCCGCAACACCAACCACCTGATCAACAACAAGACCTGGAACATCCAGTTGACCAAGACCGGCTTCACCAACGAGGCAGGCCACTGCCTGGTGATGCGCACCACGATCAACCGCAGCCCGGTCAATCTGGTGGTGCTGGACGCCTTCGGCAAGTACACCCACTTCGCCGACGCCACGCGCCTGCGACGCTGGCTGGAAACCGGCCAGGTCACTGCGATCCCGGCCGCGGCCAAGGCCTATCGCCTGCAACGCGACCGCGAGCGCGGCCTCGGCCAGCCGGTGGCCCAGGCCGTACGCTGA
    Protein Properties
    Protein Residues: 310
    Protein Molecular Weight: 34 kDa
    Protein Theoretical pI: 11.2
    Hydropathicity (GRAVY score): -0.109
    Charge at pH 7 (predicted): 20.4
    Protein Sequence: 
    >PA0869
MRNRLLSLVTLFLSLSVATAVSASPPPKAGLPLQQELASGSALVTDQNTGKVLYSRNPDLVVPIASITKLMTAMVVLDSKLPLDEVLPIAISETSEMRGVFSRVRVGSQISRRDMLLLALMSSENRAAASLAQNYPGGKNAFVKAMNAKAHALGMKNTRYVEPTGLSLHNVSTARDLTRLLVASRQYPLLSQWSTTPEKTVAFRHPNYTLGFRNTNHLINNKTWNIQLTKTGFTNEAGHCLVMRTTINRSPVNLVVLDAFGKYTHFADATRLRRWLETGQVTAIPAAAKAYRLQRDRERGLGQPVAQAVR
    References
    External Links:
    Resource Link
    Genome ID: PA0869
    Entrez Gene ID: 877694
    NCBI Protein ID: 15596066
    General Reference: PaperBLAST - Find papers about PA0869 and its homologs